类器官收获液

This 类器官收获液 enables gentle and rapid recovery of cells cultured on basement membrane matrix, while maintaining cell viability throughout the process. It is optimized for efficient organoid retrieval prior to passaging, cryopreservation, or downstream analysis.

(1) Observe organoid morphology under microscope; proceed with passaging when organoid density reaches 500 organoids per well or individual organoid diameter is 100-200 μm, to maintain optimal organoid health and proliferation rate. (2) Prepare all required reagents and consumables in advance. (3) In a biosafety cabinet, aspirate old culture medium from the plate, add pre-cooled 类器官洗涤液 (GBOR0004) for one wash. Change pipette tip, add 类器官收获液 (500 μL/well for 24-well plate), and incubate at room temperature for 5 minutes. (4) Using a 1000 μL pipette tip pre-rinsed with 漂洗液 (GBOR0006), dislodge organoids by pipetting or scraping. Collect into a 15 mL centrifuge tube, add 5 mL 类器官洗涤液, pipette organoids 5-10 times, place on ice for 10 minutes, then centrifuge at 200×g for 5 minutes at 2-8°C. Discard supernatant. (5) Choose mechanical or enzymatic dissociation method based on organoid developmental stage and morphology. (6) Centrifuge at 200×g for 5 minutes at 2-8°C, collect cell pellet, remove as much supernatant as possible, then add matrix gel or 类器官冻存液 for subsequent expansion culture or cryopreservation.